05月03 【成功案例】百邁客云 BLAST小工具 助力 孫青原老師 發(fā)表《N6-甲基腺苷測序揭示了mRNA甲基化通過調節(jié)非洲爪蟾的翻譯水平對卵母細胞減數(shù)分裂成熟和胚胎發(fā)育的影響》
中文摘要：
在非洲爪蟾的卵母細胞時期，卵母細胞積累了早期胚胎發(fā)育的母體材料。由于卵母細胞的轉錄活性在完全生長階段沉默，全基因組轉錄只在中胚層胚胎期才被重新激活，卵母細胞生長期積累的母體mRNA的翻譯應該被準確調節(jié)。以前的研究結果表明poly（A）尾長度和RNA結合元件介導卵母細胞中的RNA翻譯調節(jié)，最近發(fā)現(xiàn)RNA甲基化存在于各個翻譯調節(jié)系統(tǒng)中。本研究對完全生長的生殖囊泡（GV）階段和中期II（MII）期卵母細胞中的N6-甲基腺苷（m6A）修飾的mRNA進行測序。結果，在GV期或MII期卵母細胞中鑒定了具有m6A峰的4,207個mRNA。接著將mRNA甲基化數(shù)據(jù)與轉錄組和蛋白組數(shù)據(jù)結合分析，結果表明，盡管在RNA表達水平?jīng)]有顯著性差異，但是高甲基化的mRNA對應的蛋白質表達水平顯著低于低甲基化mRNA的蛋白表達水平，同時還發(fā)現(xiàn)低甲基化的mRNA主要富集在細胞周期和翻譯途徑，而高甲基化的mRNA主要與蛋白磷酸化有關。本文的研究結果表明卵母細胞中的mRNA甲基化可以調節(jié)卵母細胞減數(shù)分裂成熟和早期胚胎發(fā)育過程中的細胞分化和細胞分裂。
本文用了百邁客云平臺BLAST比對小工具。
英文摘要：
During the oogenesis of Xenopus laevis, oocytes accumulate maternal materials for early embryo development. As the transcription activity of oocyte is silenced at the fully-grown stage and the global genome is reactivated only by the mid- blastula embryo stage, the translation of maternal mRNAs accumulated during oocyte growth should be accurately regulated. Previous evidence has illustrated that the poly(A) tail length and RNA binding elements mediate RNA translation regulation in oocyte. Recently, RNA methylation is found to exist in various systems. In the present study, we sequenced the N6-methyladenosine (m6A) modified mRNAs in fully-grown germinal vesicle (GV) stage and metaphase II (MII) stage oocytes. As a result, we identified 4207 mRNAs with m6A peaks in the GV stage or MII stage oocytes. When we integrated the mRNA methylation data with transcriptome and proteome data, we found that the highly methylated mRNAs showed significantly lower protein levels than those of the hypomethylated mRNAs, although the RNA levels showed no significant difference. We also found that the hypomethylated mRNAs were
mainly enriched in the cell cycle and translation pathways, whereas the highly methylated mRNAs were mainly associated with the protein phosphorylation. Our results suggest that the oocyte mRNA methylation can regulate the cellular translation and cell division during oocyte meiotic maturation and early embryo development.